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The primary precursor to melanin is tyrosine, an amino acid that determines the type of melanin made based on its quantity in the polymer (it is also known as the limiting factor)

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Additionally, fresh samples eliminate potential changes to blood cells caused by damage to cell morphology during freezing (such as ice crystals puncturing cell membranes) or damage via the freezing medium [240]which is important when also trying to understand the pathophysiology of the disease

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